| 摘要: |
| 碳量子点（CQDs）以其优良的光电性能,以及环保、价廉、易得的优点而受到极大关注.以柠檬酸（CA）和三聚氰胺（Melamine）为原料,采用一步水热法成功制备了氮掺杂的碳量子点（N-CQDs）.对其形貌、组成及光谱性能进行了表征,详细研究了其电化学发光（ECL）性能,结果表明：所合成的N-CQDs在共反应剂过硫酸钾（K2S2O8）存在下能发射强且稳定的ECL信号.进一步研究显示：金纳米粒子（Au NPs）的存在会导致N-CQDs的ECL信号猝灭,而谷胱甘肽（GSH）的加入又能使其ECL信号得以恢复.根据这一特点实现了对GSH的灵敏检测,线性响应范围为1.0×10-9~1.0×10-4 mol·L-1,检出限是8.0×10-10 mol·L-1.该策略也可用于其他含巯基（-SH）的生物硫醇类物质的检测. |
| 关键词: 电化学发光（ECL） 氮掺杂碳量子点（N-CQDs） 谷胱甘肽（GSH） 金纳米粒子（Au NPs） |
| DOI：10.3969/J.ISSN.1000-5137.2023.01.006 |
| 分类号:O657.1 |
| 基金项目:上海师范大学“仪器分析”课程教学团队建设项目（304-AC9103-21-368011523） |
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| Determination of glutathione based on electrochemiluminescence of N-CQDs |
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LIU Xiangmiao, LIU Ting, YANG Minli
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College of Chemistry and Materials Science, Shanghai Normal University, Shanghai 200234, China
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| Abstract: |
| Carbon quantum dots（CQDs） have attracted great attention because of their excellent photoelectric properties, environmental friendliness, low cost, and easy availability. Nitrogen doped carbon quantum dots（N-CQDs） were prepared by one-step hydrothermal method using melamine and citric acid（CA） as raw materials. Their morphology, composition, and spectral properties were characterized with various technologies. The electrochemical luminescence（ECL） property of N-CQDs was investigated in detail. The result showed that the synthesized N-CQDs could emit strong and stable ECL signals in the presence of co-reactive potassium persulfate （K2S2O8）. Further studies showed that the presence of gold nanoparticles（Au NPs）could cause the ECL signal of N-CQDs to be quenched, and the addition of glutathione（GSH） could restore the ECL signal. Based on this principle, the sensitive detection of GSH was achieved with a linear response range of 1.0×10-9 to 1.0×10-4 mol·L-1 and a detection limit of 8.0×10-10 mol·L-1. This strategy can also be used for the detection of other mercapto（-SH） containing biothiols. |
| Key words: electrochemiluminescence（ECL） nitrogen-doped carbon quantum dots（N-CQDs） glutathione（GSH） gold nanoparticles（Au NPs） |
