Abstract: Zhaotong-Ludian fault zone is an important part of the southern boundary of Daliangshan secondary active block on the southeast margin of Qinghai-Tibet Plateau, and it is also an important seismic structure in Northeast Yunnan. In order to further understand the degrees of fault activities and late Quaternary activities, field geological and geomorphological investigations, trenching engineering, sample collections and chronologic tests were carried out. The results were as follows: ① According to the field geological and geomorphological survey results, three secondary faults in the northeast of Zhaotong-Ludian fault zone had been strongly active since the late Pleistocene, and their movement characteristics were mainly thrust and dextral strike-slip; ② The exploratory trench excavated in Xinpengzi Village of Zhaotong-Ludian fault revealed that the fault dislocated the Late Pleistocene strata (32510±30) a BP. According to the obvious directional gravel arrangements in clay layers and the disturbance of sandstone layers, it was speculated that the fault may have dislocated the Holocene strata (10880±30) a BP. To sum up, the research results are of great significance for the prediction of earthquake trend and earthquake risk in Northeast Yunnan and Sichuan-Yunnan border areas.