关键词: 泥火山； 16S rDNA； 变性梯度凝胶电泳(DGGE), DNA指纹图谱

Abstract:

16S rDNA V3 fragmentbase was separated by DGGE (denaturing gradient gel electrophoresis) and clone sequence BLAST technologies, and quantity one software was used to map the cluster analysis. The fingerprint shows that the compositions of bacteria in all the soil samples are obvriously different. The cluster analysis indicates that the structures of bacteria in the same month of soil samples in different depths are similar to each other. Specific band sequencing results show that the Pseudomonas sp is the dominant microorganisms in mud volcanic soils. The bacterial polymorphism in mud volcano soil in Xinjiang is significant, and this diversity is vulnerable to ecological, climatic and other factors, however, these factors affect the dominant microorganisms little.

Key words: mud volcano, 16S rDNA, denaturing gradient gel electrophoresis(DGGE), DNA fingerprinting