| 唐颖,王长海,李克锦,黄笛.杜氏盐藻无菌纯化研究[J].,2010,(6):871-876 |
| 杜氏盐藻无菌纯化研究 |
| Research on axenic purification of Dunaliella salina |
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| DOI：10.7511/dllgxb201006006 |
| 中文关键词: 杜氏盐藻 无菌纯化 抗生素 平板培养 |
| 英文关键词: Dunaliella salina axenic purification antibiotic plate culture |
| 基金项目:“十五”国家重点科技攻关课题资助项目(2001BA707B03). |
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| 中文摘要: |
| 首先通过杜氏盐藻及藻液内优势菌对抗生素的敏感性实验确定了氨苄青霉素、庆大霉素、头孢霉素和链霉素为该盐藻的除菌工具．采用联合加入的方法，即上述4种抗生素终浓度均为800 μg/mL，相同浓度连续混合处理3次，每次处理间隔2 d，再结合平板稀释分离法，获得无菌盐藻．带菌盐藻的生长稍快于无菌盐藻，但会较早出现老化现象；分别回加分离到带菌盐藻中的5种优势菌会促进无菌盐藻的生长．本实验为进一步的盐藻转基因以及藻菌关系等研究奠定了基础． |
| 英文摘要: |
| The appropriate antibiotics for Dunaliella salina purification were initially determined by investigating the effects of several antibiotics on the microalgae and co-occurring bacteria. With the mixture of ampicillin, gentamycin sulfate, cefalotin sodium and streptomycin added in the culture, 800 μg/mL final concentrations respectively, every two days and repeated three times, the bacteria-free algal culture was obtained by spread plate. The microalgae mixed with bacteria grew a little faster than axenic microalgae, but was aging earlier. The microalgae was stimulated to grow faster with isolated co-occurring bacteria added. The results show significance for further study on relationships between microalgae and co-occurring bacteria, as well as genetic engineering. |
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