In order to research on the effect and mechanism of the disinfection combined hydrogen peroxide with ozone on swimming pool water, hydrogen peroxide and ozone at different concentration ratios were used to sterilize the artificial pool water with high load to analyze the effects of hydrogen peroxide combined with ozone at different concentration ratio on the water quality indexes like ORP, Escherichia coli, the total bacteria and the ability of continuous disinfection. The results show that when the water temperature is kept at 25℃ and the contact time for disinfection is within 2 hours, the best concentration ratio of combined disinfection is 50 mg/L H2O2 combining with 0.20 mg/LO3, and the ORP increases by 24.46%, while the sterilizing rate of Escherichia coli and the total bacteria are respectively increased by 16.08% and 11.34% relative to only using 50 mg/L H2O2 for disinfection. After 6 hours, the total number of bacteria and Escherichia coli both can not be detected, and the three indexes all meet the requirements of the standard. It is concluded that reducing the concentration of H2O2 and adding a small amount of O3 can effectively enhance and maintain a relatively stable disinfection effect.