实验动物科学 ›› 2019, Vol. 36 ›› Issue (01): 48-.

• 研究报告 • 上一篇    下一篇

大鼠与鲫鱼肠道内含物中微生物菌群的研究

  

  1. 长春理工大学生命科学技术学院,长春 130022
  • 出版日期:2019-02-28 发布日期:2020-09-03

Study of Intestinal Flora in Rat and Crucian Carp

  • Online:2019-02-28 Published:2020-09-03

摘要: 目的 对生活环境差异较大的动物肠道内含物中微生物菌群分布规律进行研究。方法 通过提取SD大鼠与鲫鱼不同肠段的内含物菌液,用不同的选择性培养基进行分离培养,鉴定后进行平板计数。结果 大鼠肠道中乳酸杆菌所占比重高于鲫鱼肠道,其余菌落(双歧杆菌、肠球菌、大肠杆菌)比重明显小于鲫鱼肠道;大鼠和鲫鱼肠道厌氧的双歧杆菌沿肠道向下数量递增。需氧的肠球菌在鲫鱼、大鼠整个肠道中的数量级相当。结论 研究不同动物肠道的微生物信息可以为病理学、毒理学的研究提供理论信息。

关键词: 鲫鱼, 大鼠, 肠道微生物, 分离, 鉴定

Abstract: Objective The distribution of intestinal microflora in different parts of animals,intestine which living environment are quite different. Method With taking the contents of different intestinal segments of mice and crucian carp, extracting and culturing with different selective medium, and then carring out the plate count. Result It was found that the proportion of Lactobacillus in the intestine of rats was higher than that of crucian carp, and the proportion of other colonies (Bifidobacterium, Enterococcus, Escherichia coli) was significantly smaller than that of crucian carp. The distribution of intestinal anaerobic Bifidobacterium in rats and crucian carp increase in the downward direction along the gut.The magnitude of aerobic Enterococcus in the intestine of rats and crucian carp was almost same. Conclusion The inestinal microbiological information of different animals can preovide some experimental basis for the study of pathology and toxicology.

Key words: crucian carp, rat, intestinal microbiology, isolation, identification