关键词: 鸭肝炎病毒, 实时荧光定量 PCＲ, 雏鸭, 动态分布

Abstract: Abstract: Objective To investigate the dynamic distribution of duck challenge hepatitis virus strain ( 161 /79 /V) in experimentally infected ducklings using the Taqman real-time fluorescent quantitative PCＲ． Method Ducklings were infected with the 161 / 79 / V strain at 3 － day age，and the virus ＲNAs were extracted from different organs within 24 hours and quantified using the fluorescent quantitative PCＲ method． Ｒesult DHV ＲNAs were detected in heart，liver，brain，lung，pancreas，ileum，cecum，rectum，bursa and spleen 4 hours after inoculation，and the ＲNA copies continuously increased． The highest copy of the DHV ＲNA was detected in liver ( 108. 81 copies /g) 24 h after inoculation，and the ＲNA copies were somewhat low in heart，lung，kidney and spleen ( about 107 copies /g) ．Conclusion Our study indicates that the challenge DHV strain 161 / 79 / V is tissue or organ ecotropic，such as the solid organ，the immune organs and the digestive system of the infected ducklings．

Key words: DHV-1, real-time fluorescent quantitative PCＲ, duckling, dynamic distribution