Since stomatal conductance and intercellular CO2 concentration are very important physiological characteristics, a field experiment was conducted to explore the response of stomatal conductance and intercellular CO2 concentration to meteorological factors. Spring wheat (Triticum aestivum L.) was chosen for the heading and filling stage. Physiological characteristics(stomatal conductance and intercellular CO2 concentration) were measured by LI-6400 portable photosynthesis analyzer in vivo monitoring of plant., measuring meteorological factors simultaneously. The results showed that the stomatal conductance was significantly positive related to relative air humidity, while the intercellular CO2 concentration was significantly negative related to air relative humidity. Stomatal conductance and soil temperature showed a quadratic relationship, the optimum temperature was 30℃ and 33℃ in heading and filling stage. The same trend was also showed between stomatal conductance and air temperature, intercellular CO2 concentration and soil temperature, intercellular CO2 concentration and air temperature, with the optimum temperature being different.