Bacillus licheniformis strain, named B81 which could produce urease was separated from sponge Halichondria regosa. Using fermentation optimized methods Plachett-Bunman to choose the important factor of the basal medium, then the single factor experiment was used to optimize the urease production, the optimized medium was : glucose 25 g/L,peptone 12.5 g/L,beef-extracts 5 g/L,Yeast extract 6.5 g/L,KH2PO4 2 g/L,NaCl 1 g/L,urea 0.625% (W/V),Ni(NO3)2 0.00625% (W/V) , H2O 1L. Urease activity increased 170% in the optimum medium. The urease reacted best at 40℃ and pH 7.0, and had activities>90% after 30min maintenance under 50℃ and pH 7.0, and was sensetive for mental ion, such as Pb2 andCu2。